This study explores the use of forensic synthetic blood substitute (FBS) for impact pattern simulation and area of origin estimation. Ten impact patterns were created at a known origin using the FBS and were analyzed by groups of undergraduate students participating in a basic bloodstain pattern analysis course. The students selected 20 upward-moving stains from their given patterns to estimate an area of origin. Three linear trajectory models – BackTrack™, Hemospat, and Sherlock – were used to estimate each pattern's area of origin. Coordinate data from each model's analysis were compared with the known origin and between programs at the x-, y-, z-coordinates, and overall. Results from this analysis yielded estimates comparable to the known. A one-way ANOVA found no significant difference between programs at the x- (p = 0.79), y- (p = 0.84), z- (p = 0.96) coordinates, and overall (p= 0.81). These results support the practical use of the FBS as an alternative to whole blood for impact pattern simulations and area of origin estimation.