Solution–gelation chemistry has promising applications in forensic synthetic blood substitute development. This research offers a silicon-based sol–gel approach to creating stable materials that share similar rheological properties to that of whole human blood samples. Room temperature, high water content, silicon sol–gels were created using the organosilane precursors 3-glycidoxypropyltrimethoxysilane and tetraethylorthosilicate along with various concentrations of filler and pigment. Shear-thinning non-Newtonian properties were observed within most formulations of the presented materials. The effects of colloidal concentration, temperature, age and filler addition on the viscosity of the sol–gels were investigated. SEM–EDS analysis was used to identify the behaviour of the fillers within the film and support their inclusion for basic bloodstain pattern simulation. A final proposed candidate sol–gel was assessed using a previously reported passive drip simulation test on a hard, dry surface and passed. This works represents encouraging development in providing safe material alternatives to using whole human blood for forensic training and research.